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Anti-Intrinsic Factor Antibody (4J678)
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Catalog No. TMAY-02598
Alias TCN3, INF, IFMH, IF, gastric intrinsic factor (vitamin B synthesis)
Anti-Intrinsic Factor Antibody (4J678) is a Rabbit antibody targeting Intrinsic Factor. Anti-Intrinsic Factor Antibody (4J678) can be used in WB,IP.
Anti-Intrinsic Factor Antibody (4J678)
Anti-Intrinsic Factor Antibody (4J678)
😃Good
Catalog No. TMAY-02598Alias TCN3, INF, IFMH, IF, gastric intrinsic factor (vitamin B synthesis)
Anti-Intrinsic Factor Antibody (4J678) is a Rabbit antibody targeting Intrinsic Factor. Anti-Intrinsic Factor Antibody (4J678) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Human |
| Alias | TCN3, INF, IFMH, IF, gastric intrinsic factor (vitamin B synthesis) |
| Ig Type | Rabbit IgG |
| Clone | 4J678 |
| Reactivity | Human |
| Verified Activity | 1. Anti-GIF rabbit monoclonal antibody at 1:500 dilution. -Lane A: K562 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:45 kDa. -Observed band size:45 kDa. 2. GIF was immunoprecipitated using: -Lane A:0.5 mg K562 Whole Cell Lysate. -Lane B:0.5 mg Jurkat Whole Cell Lysate -0.5 µL anti-GIF rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-GIF rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 45 kDa. -Observed band size: 45 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Gastric intrinsic factor / GIF (rh Gastric intrinsic factor / GIF; TMPY-03221; P27352-1; Met1-Tyr417). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Gastric intrinsic factor / GIF Protein (TMPY-03221) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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