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Anti-Integrin alpha 5 beta 1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00976
Anti-Integrin alpha 5 beta 1 Polyclonal Antibody is a Rabbit antibody targeting Integrin alpha 5 beta 1. Anti-Integrin alpha 5 beta 1 Polyclonal Antibody can be used in WB,FCM.
Anti-Integrin alpha 5 beta 1 Polyclonal Antibody
Anti-Integrin alpha 5 beta 1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00976
Anti-Integrin alpha 5 beta 1 Polyclonal Antibody is a Rabbit antibody targeting Integrin alpha 5 beta 1. Anti-Integrin alpha 5 beta 1 Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,FCM Reactivity: Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Sheep) |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4°C). Primary Antibody (green line): Rabbit Anti-Integrin Alpha V + Beta 1 antibody (TMAB-00976), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test. 2. Sample: Lane 1: A549 (Human) Cell Lysate at 30 μg Lane 2: U251 (Human) Cell Lysate at 30 μg Lane 3: Huvec (Human) Cell Lysate at 30 μg Lane 4: SH-SY5Y (Human) Cell Lysate at 30 μg Lane 5: U2os (Human) Cell Lysate at 30 μg Lane 6: MCF-7 (Human) Cell Lysate at 30 μg Lane 7: Jurkat (Human) Cell Lysate at 30 μg Primary: Anti-Integrin Alpha V + Beta 1 (TMAB-00976) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 125 kDa Observed band size: 125 kDa |
| Application | WB,FCM |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg /test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell Membrane |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumour cells. ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. |
| Immunogen | KLH conjugated synthetic Mixed peptides 915-948/1048(human Integrin αV)+ 21-70/798(human Integrin β1) |
| Antigen Species | Human |
| Gene ID | 3685 |
| Uniprot ID | |
| Biology Area | Integrins,Integrins,Platelets,Host Virus Interaction,T Lymphocytic Lineage |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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