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Anti-Insulin Antibody (5K548)
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Catalog No. TMAB-07732
Anti-Insulin Antibody (5K548) is a Rabbit antibody targeting Insulin. Anti-Insulin Antibody (5K548) can be used in IHC-P,IHC-Fr,IF.
Anti-Insulin Antibody (5K548)
Anti-Insulin Antibody (5K548)
Copy Product Info😃Good
Catalog No. TMAB-07732
Anti-Insulin Antibody (5K548) is a Rabbit antibody targeting Insulin. Anti-Insulin Antibody (5K548) can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $151 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-Insulin Antibody (5K548) is a Rabbit antibody targeting Insulin. Anti-Insulin Antibody (5K548) can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 5K548 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Insulin Monoclonal Antibody, Unconjugated (TMAB-07732) at 1: 1500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Insulin Monoclonal Antibody, Unconjugated (TMAB-07732) at 1: 1500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Insulin Monoclonal Antibody, Unconjugated (TMAB-07732) at 1: 1000 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Insulin Monoclonal Antibody, Unconjugated (TMAB-07732) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Insulin Monoclonal Antibody, Unconjugated (TMAB-07732) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 6. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Insulin Monoclonal Antibody, Unconjugated (TMAB-07732) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:1000-5000; IHC-Fr: 1:1000-5000; IF: 1:1000-5000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma. |
| Immunogen | A synthesized peptide: human Insulin |
| Antigen Species | Human |
| Gene Name | INS |
| Gene ID | |
| Protein Name | Insulin |
| Uniprot ID | |
| Function | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. |
| Molecular Weight | Theoretical: 12 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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