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Anti-INSM2 Polyclonal Antibody
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Catalog No. TMAB-07724
Anti-INSM2 Polyclonal Antibody is a Rabbit antibody targeting INSM2. Anti-INSM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-INSM2 Polyclonal Antibody
Anti-INSM2 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-07724
Anti-INSM2 Polyclonal Antibody is a Rabbit antibody targeting INSM2. Anti-INSM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-INSM2 Polyclonal Antibody is a Rabbit antibody targeting INSM2. Anti-INSM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-INSM2 Polyclonal Antibody, Unconjugated (TMAB-07724) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Sample: U937 Cell (Human) Lysate at 40 μg Raji Cell (Human) Lysate at 40 μg Primary: Anti-INSM2 (TMAB-07724) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 63 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nuclear |
| Tissue Specificity | Expressed in heart, liver, skeletal muscle, kidney and pancreas, and, to a lesser extent, in brain, lung and spleen. In the pancreas, expressed in islet cells, including insulin- and glucagon-producing alpha- and beta-cells, but not in acinar cells (at protein level). Detected in adrenal glands, particularly in the deeper layer of the cortex (at protein level). {ECO:0000269|PubMed:21343251}. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | INSM2 is a 566 amino acid nuclear protein. INSM2 is believed to act as a growth or tumor suppressor in certain neurons and in liver cells. INSM2 contains five zinc finger C2H2-type domains which bind nucleic acids. Zinc fingers are composed of 25-30 amino acid residues and have the ability to bind about five nucleotides. C2H2-type zinc fingers have two cysteines on one extremity of the domain, and two histidines on the other extremity. These four cysteines and histidines interact with one zinc atom, resulting in the finger-like conformation of the domain. It is believed that the C2H2-type zinc fingers require the binding of a zinc atom in order for the finger to bind DNA or RNA. |
| Immunogen | KLH conjugated synthetic peptide: human INSM2 |
| Antigen Species | Human |
| Gene Name | INSM2 |
| Gene ID | |
| Protein Name | Insulinoma-associated protein 2 |
| Uniprot ID | |
| Function | INSM2 may function as a growth suppressor or tumor suppressor in liver cells and in certain neurons. |
| Molecular Weight | Theoretical: 58 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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