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Anti-INHBB Polyclonal Antibody

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Catalog No. TMAB-00969

Anti-INHBB Polyclonal Antibody is a Rabbit antibody targeting INHBB. Anti-INHBB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-INHBB Polyclonal Antibody

Anti-INHBB Polyclonal Antibody

😃Good
Catalog No. TMAB-00969
Anti-INHBB Polyclonal Antibody is a Rabbit antibody targeting INHBB. Anti-INHBB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-INHBB Polyclonal Antibody is a Rabbit antibody targeting INHBB. Anti-INHBB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep,GuineaPig)
Verified Activity
1. Sample:
A431 (Human) Cell Lysate at 40 μg
Primary: Anti-Inhibin beta B (TMAB-00969) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa
2. Sample:
Brain (Mouse) Lysate at 40 μg
Primary: Anti-Inhibin beta B (TMAB-00969) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Inhibin beta B) Polyclonal Antibody, Unconjugated (TMAB-00969) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Breast tissue lysates
Lane 3: Mouse Placenta tissue lysates
Lane 4: Mouse Liver tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Rat Breast tissue lysates
Lane 7: Human A431 cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti-Inhibin beta B (TMAB-00969) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/45 kDa
Observed band size: 46 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe inhibin beta B subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta B subunit forms a homodimer, activin B, and also joins with the beta A subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Inhibin beta B
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Gonadotrophic axis,Hypothalamic pituitary adrenal axis,Hormones
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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