Anti-INHA Polyclonal Antibody is a Rabbit antibody targeting INHA. Anti-INHA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human)

1. Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Inhibin Alpha Polyclonal Antibody, Unconjugated (TMAB-00967) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: Rat lymphoid tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Inhibin Alpha Polyclonal Antibody, Unconjugated (TMAB-00967) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample: Testis (Mouse) Lysate at 30 μg
Primary: Anti-Inhibin-α (TMAB-00967) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 25 kDa
4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Inhibin Alpha) Polyclonal Antibody, Unconjugated (TMAB-00967) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse Inhibin Alpha

Mouse

Hormones,Hormones