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Anti-Influenza A H8N4 Hemagglutinin/HA Antibody (8U127) is a Mouse antibody targeting Influenza A H8N4 Hemagglutinin/HA. Anti-Influenza A H8N4 Hemagglutinin/HA Antibody (8U127) can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $93 | 7-10 days | |
100 μL | $292 | 7-10 days |
Description | Anti-Influenza A H8N4 Hemagglutinin/HA Antibody (8U127) is a Mouse antibody targeting Influenza A H8N4 Hemagglutinin/HA. Anti-Influenza A H8N4 Hemagglutinin/HA Antibody (8U127) can be used in WB. |
Ig Type | Monoclonal Mouse IgG1 |
Clone | 8U127 |
Verified Activity | Anti-Influenza A H8N4 (A/pintail duck/Alberta/114/1979) Hemagglutinin / HA mouse monoclonal antibody at 1:1000 dilution. -Sample:Influenza A H8N4 (A/pintail duck/Alberta/114/1979) Hemagglutinin / HA -Lane A: 200ng -Lane B: 40ng -Lane C: 20ng -Secondary -Rabbit Anti-Mouse IgGF( ab )2/HRP at 1:1000 dilution.Performed under reducing conditions. |
Application | WB |
Recommended Dose | WB: 1:1000-1:5000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H8N4 Hemagglutinin (rInfluenza A virus H8N4 Hemagglutinin; Met 1-Lys 529; ABB87729.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
Conjucates | Unconjugated |
Immunogen | Recombinant Influenza A virus H8N4 Hemagglutinin Protein (TMPY-01545) |
Antigen Species | H8N4 |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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