Anti-INDOL1 Polyclonal Antibody is a Rabbit antibody targeting INDOL1. Anti-INDOL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Mouse Spleen tissue lysates
Lane 5: Rat Liver tissue lysates
Lane 6: Rat Spleen tissue lysates
Primary: Anti-INDOL1 (TMAB-07698) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 60 kD
2. Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-INDOL1 Polyclonal Antibody, Unconjugated (TMAB-07698) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human INDOL1

Human

IDO2

Indoleamine 2, 3-dioxygenase 2

Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism.