Anti-ILVBL Polyclonal Antibody is a Rabbit antibody targeting ILVBL. Anti-ILVBL Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Horse)

1. Sample:
Hcclm3 (Human) Cell Lysate at 30 μg
MCF-7 (Human) Cell Lysate at 30 μg
A431 (Human) Cell Lysate at 30 μg
Primary: Anti-ILVBL (TMAB-07686) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
2. Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-ILVBL antibody (TMAB-07686)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Sample: HepG2 Cell (Human) Lysate at 30 μg
Primary: Anti-ILVBL (TMAB-07686) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD

WB: 1:500-2000; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ILVBL

Human

ILVBL

2-hydroxyacyl-CoA lyase 2