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Anti-ILF2 Antibody (6A56) is a Rabbit antibody targeting ILF2. Anti-ILF2 Antibody (6A56) can be used in WB,IP,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-ILF2 Antibody (6A56) is a Rabbit antibody targeting ILF2. Anti-ILF2 Antibody (6A56) can be used in WB,IP,IHC,FCM. |
Synonyms | Nuclear factor of activated T-cells 45 kDa, NF45, Interleukin enhancer-binding factor 2, ILF2 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of ILF2 on different cell lysates using anti-ILF2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis tissue, Lane 2: HL-60, Lane 3: SH-SY-5Y. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ILF2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ILF2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ILF2 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ILF2 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ILF2 antibody. Counter stained with hematoxylin. 7. Flow cytometric analysis of SH-SY-5Y cells with ILF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:500; IHC: 1:50-200; IP: 1:10-20; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | NF45 (ILF2, nuclear factor of activated T cells 45 kDa) is a transcription factor that interacts with NF90 (ILF3, DRBP76) to regulate gene expression. NF45 and NF90 are proteins that belong to the double-stranded RNA-binding protein family and both are substrates for the dsRNA-activated protein kinase, PKR. The NF45/NF90 heterodimer is mainly involved in regulating IL-2 expression by binding to the antigen receptor response element (ARRE) target sequence of the IL-2 enhancer. In neuronal cells, the NF45/NF90 heterodimer can repress human rhinovirus type 2 replication by binding to a 5' untranslated region of the viral RNA that encodes the internal ribosome entry site (IRES). |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 43 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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