Anti-ILDR1 Polyclonal Antibody is a Rabbit antibody targeting ILDR1. Anti-ILDR1 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit,Sheep)

1. Sample: DU145 (Human) Cell Lysate at 30 μg
Primary: Anti-ILDR1 (TMAB-07678) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56 kD
2. Sample: Prostate (Rat) Cell Lysate at 40 μg
Primary: Anti-ILDR1 (TMAB-07678) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56 kD
3. Sample: Pancreas (Mouse) Cell Lysate at 40 μg
Primary: Anti-ILDR1 (TMAB-07678) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56 kD
4. Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ILDR1 antibody (TMAB-07678)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ILDR1

Human

ILDR1

Immunoglobulin-like domain-containing receptor 1

Putative membrane receptor.