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Anti-IL12A Polyclonal Antibody 2

Catalog No. TMAB-07597 Copy Product Info
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Anti-IL12A Polyclonal Antibody 2 is a Rabbit antibody targeting IL12A. Anti-IL12A Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

Anti-IL12A Polyclonal Antibody 2

Copy Product Info
🥰Excellent
Catalog No. TMAB-07597

Anti-IL12A Polyclonal Antibody 2 is a Rabbit antibody targeting IL12A. Anti-IL12A Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

Anti-IL12A Polyclonal Antibody 2
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-IL12A Polyclonal Antibody 2 is a Rabbit antibody targeting IL12A. Anti-IL12A Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Rat
Verified Activity
1. Sample: liver (Rat) Lysate at 40 μg
Primary: Anti-IL-12 (TMAB-07597) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 24 kD
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IL-12) Polyclonal Antibody, Unconjugated (TMAB-07597) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research Backgroundbs-0767P is one synthetic peptide derived from mouse IL-12. IL-12 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: rat IL-12
Antigen Species
Rat
Gene Name
IL12A
Gene ID
3592
Protein Name
Interleukin-12 subunit alpha
Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Chemical Properties
Molecular WeightTheoretical: 22 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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