Anti-IL12A Polyclonal Antibody is a Rabbit antibody targeting IL12A. Anti-IL12A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human

1. Sample: MDA-MB-231 (Human) Cell Lysate at 40 μg
Primary: Anti-IL12 (TMAB-07598) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 34 kD
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated (TMAB-07598) 1:500, overnight at 4℃; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37℃. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
3. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated (TMAB-07598) 1:500, overnight at 4℃; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37℃. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
4. Sample: U251 Cell Lysate at 30 μg
Primary: Anti-IL12 (TMAB-07598) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 34 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human IL-12

Human

IL12A

Interleukin-12 subunit alpha

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.