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Anti-IL-6 Antibody (9Y569)
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Catalog No. TMAY-00751
Alias interleukin 6
Anti-IL-6 Antibody (9Y569) is a Mouse antibody targeting IL-6. Anti-IL-6 Antibody (9Y569) can be used in WB,IHC-P.
Anti-IL-6 Antibody (9Y569)
Anti-IL-6 Antibody (9Y569)
😃Good
Catalog No. TMAY-00751Alias interleukin 6
Anti-IL-6 Antibody (9Y569) is a Mouse antibody targeting IL-6. Anti-IL-6 Antibody (9Y569) can be used in WB,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $69 | 7-10 days | |
| 100 μL | $198 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-IL-6 Antibody (9Y569) is a Mouse antibody targeting IL-6. Anti-IL-6 Antibody (9Y569) can be used in WB,IHC-P. |
| Alias | interleukin 6 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 9Y569 |
| Reactivity | Human |
| Verified Activity | 1. Anti-IL6 mouse monoclonal antibody at 1:500 dilution. -Lane A: A549 Whole Cell Lysate. -Lane B: Hela Whole Cell Lysate. -Lane C: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:24 kDa. -Observed band size:30 kDa. 2. Immunochemical staining of human IL6 in human spleen with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections. 3. Immunochemical staining of human IL6 in human liver with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections. |
| Application | WB,IHC-P |
| Recommended Dose | WB: 1:500-1:2000; IHC-P: 1:30-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-6 (rh IL-6; TMPY-00863; NP_000591.1; Val30-Met212). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human IL-6 Protein (TMPY-00863) |
| Antigen Species | Human |
| Biology Area | Neuroinflammation, Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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