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Anti-IL-33 Antibody (6H166)
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Catalog No. TMAY-00705
Anti-IL-33 Antibody (6H166) is a Mouse antibody targeting IL-33. Anti-IL-33 Antibody (6H166) can be used in WB,IP.
Anti-IL-33 Antibody (6H166)
Anti-IL-33 Antibody (6H166)
😃Good
Catalog No. TMAY-00705
Anti-IL-33 Antibody (6H166) is a Mouse antibody targeting IL-33. Anti-IL-33 Antibody (6H166) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-IL-33 Antibody (6H166) is a Mouse antibody targeting IL-33. Anti-IL-33 Antibody (6H166) can be used in WB,IP. |
| Ig Type | Monoclonal Mouse IgG2a |
| Clone | 6H166 |
| Reactivity | Human |
| Verified Activity | 1. Anti-IL-33 mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: K562 Whole Cell Lysate. -Lane C: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:31 kDa. -Observed band size:25 kDa. 2. IL-33 was immunoprecipitated using: -Lane A:0.5 mg HeLa，K562，jurkat Whole Cell Lysate. -2 µL anti-IL-33 mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-IL-33 mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 31 kDa. -Observed band size:25 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 0.5-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-33 (rh IL-33; TMPY-00861; NP_254274.1; Ser112-Thr270). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human IL-33 Protein (TMPY-00861) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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