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Anti-IL-32 alpha/IL32A Antibody (2S286)

😃Good
Catalog No. TMAY-01582
Alias TAIFd, TAIFc, TAIFb, TAIFa, TAIF, NK4, IL32α, IL-32gamma, IL-32delta, IL-32beta, IL-32alpha, IL-32A, IL32A, IL-32 α, IL32 α, IL-32 alpha, IL32 alpha, IL32 A

Anti-IL-32 alpha/IL32A Antibody (2S286) is a Mouse antibody targeting IL-32 alpha/IL32A. Anti-IL-32 alpha/IL32A Antibody (2S286) can be used in WB.

Anti-IL-32 alpha/IL32A Antibody (2S286)

Anti-IL-32 alpha/IL32A Antibody (2S286)

😃Good
Catalog No. TMAY-01582Alias TAIFd, TAIFc, TAIFb, TAIFa, TAIF, NK4, IL32α, IL-32gamma, IL-32delta, IL-32beta, IL-32alpha, IL-32A, IL32A, IL-32 α, IL32 α, IL-32 alpha, IL32 alpha, IL32 A
Anti-IL-32 alpha/IL32A Antibody (2S286) is a Mouse antibody targeting IL-32 alpha/IL32A. Anti-IL-32 alpha/IL32A Antibody (2S286) can be used in WB.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-IL-32 alpha/IL32A Antibody (2S286) is a Mouse antibody targeting IL-32 alpha/IL32A. Anti-IL-32 alpha/IL32A Antibody (2S286) can be used in WB.
AliasTAIFd, TAIFc, TAIFb, TAIFa, TAIF, NK4, IL32α, IL-32gamma, IL-32delta, IL-32beta, IL-32alpha, IL-32A, IL32A, IL-32 α, IL32 α, IL-32 alpha, IL32 alpha, IL32 A
Ig Type
Monoclonal Mouse IgG2a
Clone
2S286
Reactivity
Human
Verified Activity
Anti-IL32A mouse monoclonal antibody at 1:500 dilution.

-Lane A: Jurkat Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:26 kDa.

-Observed band size:30 kDa(We are unsure as to the identity of these extra bands.)
Application
WB
Recommended Dose
WB: 1:500-1:1000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-32 alpha / IL32A (rh IL-32 alpha / IL32A; TMPY-01033; NP_001012651.1; Met1-Lys131). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundIL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human IL-32 alpha / IL32A Protein (TMPY-01033)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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