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Anti-IL-3 Antibody (8Y609)
😃Good
Catalog No. TMAY-03784
Alias PSF, MCGF, interleukin 3, Il-3, HCGF, Csfmu, BPA
Anti-IL-3 Antibody (8Y609) is a Mouse antibody targeting IL-3. Anti-IL-3 Antibody (8Y609) can be used in WB,IP.
Anti-IL-3 Antibody (8Y609)
Anti-IL-3 Antibody (8Y609)
😃Good
Catalog No. TMAY-03784Alias PSF, MCGF, interleukin 3, Il-3, HCGF, Csfmu, BPA
Anti-IL-3 Antibody (8Y609) is a Mouse antibody targeting IL-3. Anti-IL-3 Antibody (8Y609) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, IP Reactivity: Rat |
| Alias | PSF, MCGF, interleukin 3, Il-3, HCGF, Csfmu, BPA |
| Ig Type | Mouse IgG1 |
| Clone | 8Y609 |
| Reactivity | Rat |
| Verified Activity | 1. Anti-IL3 mouse monoclonal antibody at 1:500 dilution. -Lane A: Rat heart tissue lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:17 kDa. -Observed band size:17 kDa. 2. IL3 was immunoprecipitated using: -Lane A:0.5 mg A431 Whole Cell Lysate. -4 µL anti-IL3 mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-IL3 mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 17 kDa. -Observed band size:18 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 5-10 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat IL3 (rR IL3; TMPY-02772; NP_113701.1; Met1-Cys169). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Rat IL3 Protein (TMPY-02772) |
| Antigen Species | Rat |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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