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Anti-IL-1RA Antibody (9N548)

😃Good
Catalog No. TMAY-00304
Alias interleukin 1 receptor antagonist

Anti-IL-1RA Antibody (9N548) is a Mouse antibody targeting IL-1RA. Anti-IL-1RA Antibody (9N548) can be used in FCM,ICC/IF.

Anti-IL-1RA Antibody (9N548)

Anti-IL-1RA Antibody (9N548)

😃Good
Catalog No. TMAY-00304Alias interleukin 1 receptor antagonist
Anti-IL-1RA Antibody (9N548) is a Mouse antibody targeting IL-1RA. Anti-IL-1RA Antibody (9N548) can be used in FCM,ICC/IF.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-IL-1RA Antibody (9N548) is a Mouse antibody targeting IL-1RA. Anti-IL-1RA Antibody (9N548) can be used in FCM,ICC/IF.
Aliasinterleukin 1 receptor antagonist
Ig Type
Monoclonal Mouse IgG2b
Clone
9N548
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human IL1Ra in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human IL1Ra monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.

2. Flow cytometric analysis of Human IL1RA expression on A431 cells. Cells were stained with purified anti-Human IL1RA, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
FCM,ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-1RA (rh IL-1RA; TMPY-00853; NP_776214.1; Arg26-Glu177). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundInterleukin-1 receptor antagonist (IL-1RA) also known as IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A), and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of this protein-encoding gene is reported to be associated with an increased risk of osteoporotic fractures and gastric cancer. IL-1RA/IL1RN may inhibit the activity of IL-1 by binding to its receptor and it has no IL-1 like activity. Genetic variation in IL-1RA/IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in IL-1RA/IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) which is also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis, and pustulosis from birth.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human IL-1RA protein (TMPY-00853)
Antigen Species
Human
Biology Area
Neuroinflammation
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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