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Anti-IL-1 alpha/IL-1A Polyclonal Antibody
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Catalog No. TMAB-00931
Anti-IL-1 alpha/IL-1A Polyclonal Antibody is a Rabbit antibody targeting IL-1 alpha/IL-1A. Anti-IL-1 alpha/IL-1A Polyclonal Antibody can be used in WB,FCM.
Anti-IL-1 alpha/IL-1A Polyclonal Antibody
Anti-IL-1 alpha/IL-1A Polyclonal Antibody
😃Good
Catalog No. TMAB-00931
Anti-IL-1 alpha/IL-1A Polyclonal Antibody is a Rabbit antibody targeting IL-1 alpha/IL-1A. Anti-IL-1 alpha/IL-1A Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,FCM Reactivity: Human,Mouse (predicted:Rat) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-IL-1 Alpha (TMAB-00931) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/31 kDa Observed band size: 31 kDa 2. Sample: Lver (Mouse) Lysate at 40 μg Primary: Anti-IL-1 Alpha (TMAB-00931) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/31 kDa Observed band size: 31 kDa 3. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-IL-1 Alpha antibody (TMAB-00931) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| Application | WB,FCM |
| Recommended Dose | WB: 1:500-2000; FCM: 1ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence. |
| Immunogen | KLH conjugated synthetic peptide: rat IL-1 Alpha |
| Antigen Species | Rat |
| Gene ID | 24493 |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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