Anti-IL-1 alpha/IL-1A Polyclonal Antibody

Anti-IL-1 alpha/IL-1A Polyclonal Antibody is a Rabbit antibody targeting IL-1 alpha/IL-1A. Anti-IL-1 alpha/IL-1A Polyclonal Antibody can be used in FCM,WB.

IgG

Human,Mouse (predicted:Rat)

1. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-IL-1 Alpha (TMAB-00931) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/31 kDa
Observed band size: 31 kDa
2. Sample:
Lver (Mouse) Lysate at 40 μg
Primary: Anti-IL-1 Alpha (TMAB-00931) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/31 kDa
Observed band size: 31 kDa
3. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-IL-1 Alpha antibody (TMAB-00931)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: rat IL-1 Alpha

Rat

IL1A

Interleukin-1 alpha

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.