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Anti-IKZF1 Antibody (8M494)

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Catalog No. TMAC-02114

Anti-IKZF1 Antibody (8M494) is a Rabbit antibody targeting IKZF1. Anti-IKZF1 Antibody (8M494) can be used in FCM,IHC,WB.

Anti-IKZF1 Antibody (8M494)

Anti-IKZF1 Antibody (8M494)

😃Good
Catalog No. TMAC-02114
Anti-IKZF1 Antibody (8M494) is a Rabbit antibody targeting IKZF1. Anti-IKZF1 Antibody (8M494) can be used in FCM,IHC,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-IKZF1 Antibody (8M494) is a Rabbit antibody targeting IKZF1. Anti-IKZF1 Antibody (8M494) can be used in FCM,IHC,WB.
Ig Type
IgG
Clone
8M494
Reactivity
Human
Verified Activity
1. Western blot analysis of Ikaros on human thymus tissue lysate using anti-Ikaros antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ikaros antibody. Counter stained with hematoxylin.
5. Flow cytometric analysis of Daudi cells with Ikaros antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:500-2000; IHC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundIkaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Similarly, Helios expression is detected primarily in T cells and in the earliest embryonic hemopoietic precursors and in adult stem cells. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and lL-2 mediated signaling events.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 57/48 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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