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Anti-IKBKB Polyclonal Antibody

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Catalog No. TMAB-00930
Alias Serine/threonine protein kinase IKBKB, Nuclear factor NF-kappa-B inhibitor kinase beta (NFKBIKB), Inhibitor of nuclear factor kappa-B kinase subunit beta, IKK-beta, IKK-B, IKKB, IkBKB, IKBKB, I-kappa-B-kinase beta, I-kappa-B kinase 2 (IKK-2;IKK2)

Anti-IKBKB Polyclonal Antibody is a Rabbit antibody targeting IKBKB. Anti-IKBKB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-IKBKB Polyclonal Antibody

Anti-IKBKB Polyclonal Antibody

😃Good
Catalog No. TMAB-00930Alias Serine/threonine protein kinase IKBKB, Nuclear factor NF-kappa-B inhibitor kinase beta (NFKBIKB), Inhibitor of nuclear factor kappa-B kinase subunit beta, IKK-beta, IKK-B, IKKB, IkBKB, IKBKB, I-kappa-B-kinase beta, I-kappa-B kinase 2 (IKK-2;IKK2)
Anti-IKBKB Polyclonal Antibody is a Rabbit antibody targeting IKBKB. Anti-IKBKB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Instruction Manual of Detection Antibody

Product Introduction

Bioactivity
Description
Anti-IKBKB Polyclonal Antibody is a Rabbit antibody targeting IKBKB. Anti-IKBKB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
AliasSerine/threonine protein kinase IKBKB, Nuclear factor NF-kappa-B inhibitor kinase beta (NFKBIKB), Inhibitor of nuclear factor kappa-B kinase subunit beta, IKK-beta, IKK-B, IKKB, IkBKB, IKBKB, I-kappa-B-kinase beta, I-kappa-B kinase 2 (IKK-2;IKK2)
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. 25 μg total protein per Lane of various lysates probed with IKK beta polyclonal antibody, unconjugated (TMAB-00930) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKK beta Polyclonal Antibody, Unconjugated (TMAB-00930) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKK beta Polyclonal Antibody, Unconjugated (TMAB-00930) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Application
WB
IHC-P
IHC-Fr
ICC/IF
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Membrane raft. Note=Colocalized with DPP4 in membrane rafts.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundIKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK1.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human IKK beta
Antigen Species
Human
Gene ID
3551
Uniprot ID
O14920
Biology Area
IKK,Inflammatory mediators,IKK,TLR Signaling,Other Kinases,NFkB Pathway
Chemical Properties
Molecular WeightTheoretical: 87 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Nuclear factor NF-kappa-B inhibitor kinase β (NFKBIKB)Nuclear factor NF-kappa-B inhibitor kinase b (NFKBIKB)NFKBIKBInhibitor of nuclear factor kappa-B kinase subunit βInhibitor of nuclear factor kappa-B kinase subunit bIKK-βIKKβIKK-2IKK2I-kappa-B-kinase βI-kappa-B-kinase b
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