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Anti-IKB epsilon Polyclonal Antibody

Catalog No. TMAB-07573 Copy Product Info
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Anti-IKB epsilon Polyclonal Antibody is a Rabbit antibody targeting IKB epsilon. Anti-IKB epsilon Polyclonal Antibody can be used in WB.

Anti-IKB epsilon Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-07573

Anti-IKB epsilon Polyclonal Antibody is a Rabbit antibody targeting IKB epsilon. Anti-IKB epsilon Polyclonal Antibody can be used in WB.

Anti-IKB epsilon Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2237-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-IKB epsilon Polyclonal Antibody is a Rabbit antibody targeting IKB epsilon. Anti-IKB epsilon Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat)
Verified Activity
1. Sample: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-IKB epsiloN (TMAB-07573) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
2. Sample: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-IKB epsiloN (TMAB-07573) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
Tissue SpecificityHighly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundNFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT, AP1 (JUN), and NFAT.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human IKB epsilon
Antigen Species
Human
Gene Name
NFKBIE
Gene ID
4794
Protein Name
NF-kappa-B inhibitor epsilon
Uniprot ID
O00221
Function
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Chemical Properties
Molecular WeightTheoretical: 53 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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