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Anti-IkB alpha/NFKBIA Antibody (5M346)
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Catalog No. TMAB-00927
Alias nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α
Anti-IkB alpha/NFKBIA Antibody (5M346) is a Mouse antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Antibody (5M346) can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.
Anti-IkB alpha/NFKBIA Antibody (5M346)
Anti-IkB alpha/NFKBIA Antibody (5M346)
😃Good
Catalog No. TMAB-00927Alias nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α
Anti-IkB alpha/NFKBIA Antibody (5M346) is a Mouse antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Antibody (5M346) can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-IkB alpha/NFKBIA Antibody (5M346) is a Mouse antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Antibody (5M346) can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB. |
| Synonyms | nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α |
| Ig Type | IgG1 |
| Clone | 5M346 |
| Reactivity | Human,Rat |
| Verified Activity | 1. Sample: Jurkat (Human) Cell Lysate at 30 μg Primary: Anti-IKB alpha (TMAB-00927) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa 2. Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 μg Lane 2: Hela (Human) Cell Lysate at 30 μg Lane 3: Siha (Human) Cell Lysate at 30 μg Primary: Anti-IKB alpha (TMAB-00927) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa 3. Sample: 293t (Human) Cell Lysate at 30 μg Primary: Anti-IKB alpha (TMAB-00927) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa 4. Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IKB alpha) Monoclonal Antibody, Unconjugated (TMAB-00927) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 5. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (IKB alpha) monoclonal Antibody, unconjugated (TMAB-00927) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ICC/IF: 1:50-1:200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. |
| Tissue Specificity | Highly expressed in lymph node, thymus followed by liver, brain, muscle, kidney, gastrointestinal and reproductive tract. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Immunogen | Recombinant Protein: human IKB alpha Protein |
| Antigen Species | Human |
| Gene Name | NFKBIA |
| Gene ID | |
| Protein Name | NF-kappa-B inhibitor alpha |
| Uniprot ID | |
| Biology Area | NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway |
| Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| Molecular Weight | Theoretical: 36 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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