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Anti-IkB alpha/NFKBIA Antibody (5M346)
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Catalog No. TMAB-00927
Anti-IkB alpha/NFKBIA Antibody (5M346) is a Mouse antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Antibody (5M346) can be used in WB,IHC-P,IHC-Fr,IF,ICC/IF.
Anti-IkB alpha/NFKBIA Antibody (5M346)
Anti-IkB alpha/NFKBIA Antibody (5M346)
😃Good
Catalog No. TMAB-00927
Anti-IkB alpha/NFKBIA Antibody (5M346) is a Mouse antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Antibody (5M346) can be used in WB,IHC-P,IHC-Fr,IF,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-IkB alpha/NFKBIA Antibody (5M346) is a Mouse antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Antibody (5M346) can be used in WB,IHC-P,IHC-Fr,IF,ICC/IF. |
| Ig Type | IgG1 |
| Clone | 5M346 |
| Reactivity | Human,Rat |
| Verified Activity | 1. Sample: Jurkat (Human) Cell Lysate at 30 μg Primary: Anti-IKB alpha (TMAB-00927) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa 2. Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 μg Lane 2: Hela (Human) Cell Lysate at 30 μg Lane 3: Siha (Human) Cell Lysate at 30 μg Primary: Anti-IKB alpha (TMAB-00927) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa 3. Sample: 293t (Human) Cell Lysate at 30 μg Primary: Anti-IKB alpha (TMAB-00927) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa 4. Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IKB alpha) Monoclonal Antibody, Unconjugated (TMAB-00927) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 5. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (IKB alpha) monoclonal Antibody, unconjugated (TMAB-00927) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. |
| Application | WB,IHC-P,IHC-Fr,IF,ICC/IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ICC/IF: 1:50-1:200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Immunogen | Recombinant Protein: human IKB alpha Protein |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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