Anti-IGFBP6 Polyclonal Antibody is a Rabbit antibody targeting IGFBP6. Anti-IGFBP6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-IGFBP6 Polyclonal Antibody, Unconjugated (TMAB-07556) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Brain (Rat) Lysate at 40 μg
Intestine (Mouse) Lysate at 40 μg
Primary: Anti-IGFBP6 (TMAB-07556) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
3. Sample: muscle (Mouse) Lysate at 40 μg
Primary: Anti-IGFBP6 (TMAB-07556) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 36 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human IGFBP6

Human

IGFBP6

Insulin-like growth factor-binding protein 6

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.