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Anti-IGFBP2 Polyclonal Antibody
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Catalog No. TMAB-00922
Alias mIGFBP-2, IGF-BP53, IGFBP-2, IGFBP2, IBP-2, BP2, AI255832
Anti-IGFBP2 Polyclonal Antibody is a Rabbit antibody targeting IGFBP2. Anti-IGFBP2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-IGFBP2 Polyclonal Antibody
Anti-IGFBP2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00922Alias mIGFBP-2, IGF-BP53, IGFBP-2, IGFBP2, IBP-2, BP2, AI255832
Anti-IGFBP2 Polyclonal Antibody is a Rabbit antibody targeting IGFBP2. Anti-IGFBP2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-IGFBP2 Polyclonal Antibody is a Rabbit antibody targeting IGFBP2. Anti-IGFBP2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | mIGFBP-2, IGF-BP53, IGFBP-2, IGFBP2, IBP-2, BP2, AI255832 |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat) |
| Verified Activity | 1. Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-IGFBP2 Polyclonal Antibody, Unconjugated (TMAB-00922) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-IGFBP2 Polyclonal Antibody, Unconjugated (TMAB-00922) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lane 1: Human MOLT4 cell lysates Lane 2: Human MCF-7 cell lysates Primary: Anti-IGFBP2 (TMAB-00922) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | IGFBP2 (insulin-like growth factor binding protein 2) is a member of the ISGBP family which bind various IGFs. IGFBP2 is overexpressed in a wide spectrum of other cancers, including glioma, prostate cancer, synovial sarcoma, neuroblastoma, colon cancer, adrenocortical cancer, lung cancer, Wilms' tumor, and hepatoblastoma. The overexpression of IGFBP2 also correlates with the aggressiveness of some tumors. IGFBP2 activates the expression of matrix metalloprotease 2, which contributes to cell invasiveness |
| Immunogen | KLH conjugated synthetic peptide: human IGFBP2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Insulin and insulin-like,Extracellular matrix,Insulin / Insulin-like |
| Molecular Weight | Theoretical: 36 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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