Anti-IGFBP2 Antibody (5U138)

Anti-IGFBP2 Antibody (5U138) is a Rabbit antibody targeting IGFBP2. Anti-IGFBP2 Antibody (5U138) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

5U138

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with IGFBP2 monoclonal antibody, unconjugated (TMAB-07550) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IGFBP2 Monoclonal Antibody, Unconjugated (TMAB-07550) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IGFBP2 Monoclonal Antibody, Unconjugated (TMAB-07550) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IGFBP2 Monoclonal Antibody, Unconjugated (TMAB-07550) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IGFBP2 Monoclonal Antibody, Unconjugated (TMAB-07550) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

A synthesized peptide: human IGFBP2

Human

IGFBP2

Insulin-like growth factor-binding protein 2

Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.