Anti-IGFBP-3 Polyclonal Antibody is a Rabbit antibody targeting IGFBP-3. Anti-IGFBP-3 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.

IgG

Rat (predicted:Human,Mouse)

1. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IGFBP3 Polyclonal Antibody, Unconjugated (TMAB-00923) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IGFBP3 Polyclonal Antibody, Unconjugated (TMAB-00923) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human IGFBP3

Human

IGFBP3

Insulin-like growth factor-binding protein 3

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