Antibody Type: Rabbit Polyclonal

Application: IHC-P,IHC-Fr,IF

Reactivity: Human,Rat (predicted:Mouse)

IgG

Human,Rat (predicted:Mouse)

1. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IGFBP3 Polyclonal Antibody, Unconjugated (TMAB-00923) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IGFBP3 Polyclonal Antibody, Unconjugated (TMAB-00923) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

IHC-P,IHC-Fr,IF

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human IGFBP3

Human

3486

Insulin and insulin-like,Angiogenic inhibitory factors,Angiogenic Factors,Apoptosis,Diabetes associated,Associated Proteins,Growth factors and hormones ELISA kits,Insulin / Insulin-like