Anti-IGF1R/CD221 Polyclonal Antibody is a Rabbit antibody targeting IGF1R/CD221. Anti-IGF1R/CD221 Polyclonal Antibody can be used in FCM.

IgG

Human,Mouse,Dog (predicted:Rat,Pig,Cow,Horse,Rabbit,Sheep)

1. Positive control: (mo) splenocytes (2% Paraformaldehyde-fixed)
Isotype Control Antibody
Antibody: Rabbit IgG;
Supplier: Cell Signaling Technology;
Dilution: 1 μg in 100 μL 1 X PBS containing 0.5% BSA
Secondary Antibody
Antibody: Goat anti-rabbit IgG-FITC;
Supplier: Cell Signaling Technology;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody
Supplier: Bioss;
Supplier catalog number: TMAB-00918;
Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA
2. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-IGF1R antibody (TMAB-00918)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human IGF-1R alpha chain

Human

Insulin / Insulin-like,Aging,Insulin and insulin-like