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Anti-IFN-alpha 2b/IFNA2 Polyclonal Antibody

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Catalog No. TMAB-00912

Anti-IFN-alpha 2b/IFNA2 Polyclonal Antibody is a Rabbit antibody targeting IFN-alpha 2b/IFNA2. Anti-IFN-alpha 2b/IFNA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-IFN-alpha 2b/IFNA2 Polyclonal Antibody

Anti-IFN-alpha 2b/IFNA2 Polyclonal Antibody

😃Good
Catalog No. TMAB-00912
Anti-IFN-alpha 2b/IFNA2 Polyclonal Antibody is a Rabbit antibody targeting IFN-alpha 2b/IFNA2. Anti-IFN-alpha 2b/IFNA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-IFN-alpha 2b/IFNA2 Polyclonal Antibody is a Rabbit antibody targeting IFN-alpha 2b/IFNA2. Anti-IFN-alpha 2b/IFNA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IFN-Alpha 2b/Interferon alpha 2/INFA2 Polyclonal Antibody, Unconjugated (TMAB-00912) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Interferon alpha 2b) Polyclonal Antibody, Unconjugated (TMAB-00912) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: Recombinant human Interferon alpha 2 protein, N-His
Primary: Anti-Interferon alpha 2b (TMAB-00912) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 16 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundInterferon alpha 2b is one of the known 23 different variants of IFN-alpha. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. Interferon alpha 2b is a potent immune modulator. It can either inhibit or enhance immune cell activity within the tightly regulated microenvironment of inflammation, depending upon the concentration of the cytokine and the activation stage of the cell. Interferon alpha 2B is currently used as an anticancer drug due to it's antiproliferative activity.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human IFN alpha 2b
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Interferons,Interferons
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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