Antibody Type: Rabbit Monoclonal

Application: WB, ICC/IF, IP

Reactivity: Human

Rabbit IgG

6D104

Human

1. Anti-IFITM1 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: K562 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:14 kDa.
-Observed band size:18 kDa.
2. CD225 was immunoprecipitated using:
-Lane A:0.5 mg K562 Whole Cell Lysate.
-2 µL anti-CD225 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-CD225 rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 15 kDa.
-Observed band size: 15 kDa.
3. Immunofluorescence staining of IFITM1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human IFITM1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

WB,ICC/IF,IP

WB: 1:500-1:2000; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate

Monoclonal

Unconjugated

A synthetic peptide: center region of the Human IFITM1

Human