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Anti-IFITM1 Antibody (6D104) is a Rabbit antibody targeting IFITM1. Anti-IFITM1 Antibody (6D104) can be used in WB,ICC/IF,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $270 | 7-10 days |
Description | Anti-IFITM1 Antibody (6D104) is a Rabbit antibody targeting IFITM1. Anti-IFITM1 Antibody (6D104) can be used in WB,ICC/IF,IP. |
Ig Type | Monoclonal Rabbit IgG |
Clone | 6D104 |
Reactivity | Human |
Verified Activity | 1. Anti-IFITM1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: K562 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:14 kDa. -Observed band size:18 kDa. 2. CD225 was immunoprecipitated using: -Lane A:0.5 mg K562 Whole Cell Lysate. -2 µL anti-CD225 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CD225 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 15 kDa. -Observed band size: 15 kDa. 3. Immunofluorescence staining of IFITM1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human IFITM1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
Application | WB,ICC/IF,IP |
Recommended Dose | WB: 1:500-1:2000; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human IFITM1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide corresponding to the center region of the Human IFITM1 |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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