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Anti-Iduronate 2 sulfatase Polyclonal Antibody

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Catalog No. TMAB-07502

Anti-Iduronate 2 sulfatase Polyclonal Antibody is a Rabbit antibody targeting Iduronate 2 sulfatase. Anti-Iduronate 2 sulfatase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Iduronate 2 sulfatase Polyclonal Antibody

Anti-Iduronate 2 sulfatase Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-07502
Anti-Iduronate 2 sulfatase Polyclonal Antibody is a Rabbit antibody targeting Iduronate 2 sulfatase. Anti-Iduronate 2 sulfatase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2207-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Iduronate 2 sulfatase Polyclonal Antibody is a Rabbit antibody targeting Iduronate 2 sulfatase. Anti-Iduronate 2 sulfatase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Dog,Cow,Sheep)
Verified Activity
1. Sample: 293T Cell (Human) Lysate at 30 μg
Primary: Anti-Iduronate 2 sulfatase (TMAB-07502) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 62 kD
2. Sample: Hela Cell (Human) Lysate at 30 μg
Primary: Anti-Iduronate 2 sulfatase (TMAB-07502) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 62 kD
3. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Iduronate 2 sulfatase) Polyclonal Antibody, Unconjugated (TMAB-07502) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationLysosome.
Tissue SpecificityLiver, kidney, lung, and placenta.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene belongs to the sulfatase family, is localized to the lysosome, and is involved in lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease, mucopolysaccharidosis type II, also known as Hunter syndrome. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Aug 2013]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Iduronate 2 sulfatase
Antigen Species
Human
Gene Name
IDS
Gene ID
Protein Name
Iduronate 2-sulfatase
Uniprot ID
Function
Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.
Chemical Properties
Molecular WeightTheoretical: 47 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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