Anti-ICOS Antibody (4Y416) is a Rabbit antibody targeting ICOS. Anti-ICOS Antibody (4Y416) can be used in WB,ICC/IF,FCM.

IgG

4Y416

Human,Mouse

1. 4% Paraformaldehyde-fixed HL-60 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (ICOS) monoclonal Antibody, unconjugated (TMAB-07483) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The HL-60 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-ICOS antibody (TMAB-07483): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 25 μg total protein per lane of various lysates (see on figure) probed with ICOS monoclonal antibody, unconjugated (TMAB-07483) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:1000-2000; ICC/IF: 1:50-200; FCM: 1μg/Test

Monoclonal

A synthesized peptide: human ICOS

Human

ICOS

Inducible T-cell costimulator

Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).