Anti-ICAM2 Polyclonal Antibody is a Rabbit antibody targeting ICAM2. Anti-ICAM2 Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat)

1. Blank control: Jurkat cells (blue). Primary Antibody: Rabbit Anti-CD102 antibody (TMAB-07475), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-07475, 1 μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA+1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
2. Sample: JurkaT (Human) Cell Lysate at 30 μg
Primary: Anti-CD102 (TMAB-07475) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
3. Sample:
Molt-4 (Human) Cell Lysate at 30 μg
k562 (human) Cell Lysate at 30 μg
Primary: Anti-ICAM-2/CD102 (TMAB-07475) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 31 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ICAM-2

Human

ICAM2

Intercellular adhesion molecule 2

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.