Anti-ICAM-1/CD54 Polyclonal Antibody is a Rabbit antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (TMAB-00905) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (TMAB-00905) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (TMAB-00905) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (TMAB-00905) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (TMAB-00905) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (TMAB-00905) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Uterine body cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1 Polyclonal Antibody, Unconjugated (TMAB-00905) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. 25 μg total protein per Lane of various lysates probed with ICAM1 polyclonal antibody, unconjugated (TMAB-00905) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; WB: 1:500-2000

Polyclonal

KLH conjugated synthetic peptide: human CD54

Human

ICAM1

Intercellular adhesion molecule 1

Endothelial Cell Markers,Cell adhesion molecules,Regulatory T Cells,Adhesion,SARS Coronavirus,B Lymphocytic Lineage,T Lymphocytic Lineage

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.