Anti-Hyaluronidase3 Polyclonal Antibody is a Rabbit antibody targeting Hyaluronidase3. Anti-Hyaluronidase3 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Pig,Sheep)

1. Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-Hyaluronidase3 antibody (TMAB-07463)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample: Mouse (Kidney) Lysate at 40 μg
Primary: Anti-Hyaluronidase3 (TMAB-07463) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
3. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Stomach tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Stomach tissue lysates
Primary: Anti-Hyaluronidase3 (TMAB-07463) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 53 kDa

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Hyaluronidase3

Human

HYAL3

Hyaluronidase-3