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Anti-Human Lambda Light Chain Antibody (8H783)

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Catalog No. TMAB-07448

Anti-Human Lambda Light Chain Antibody (8H783) is a Mouse antibody targeting Human Lambda Light Chain. Anti-Human Lambda Light Chain Antibody (8H783) can be used in IHC-P,WB,IHC-Fr,IF.

Anti-Human Lambda Light Chain Antibody (8H783)

Anti-Human Lambda Light Chain Antibody (8H783)

Copy Product Info
😃Good
Catalog No. TMAB-07448
Anti-Human Lambda Light Chain Antibody (8H783) is a Mouse antibody targeting Human Lambda Light Chain. Anti-Human Lambda Light Chain Antibody (8H783) can be used in IHC-P,WB,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2227-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Human Lambda Light Chain Antibody (8H783) is a Mouse antibody targeting Human Lambda Light Chain. Anti-Human Lambda Light Chain Antibody (8H783) can be used in IHC-P,WB,IHC-Fr,IF.
Ig Type
IgG1
Clone
8H783
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (TMAB-07448) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (TMAB-07448) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. 25 μg total protein per lane of various lysates (see on figure) probed with Human Lambda Light Chain monoclonal antibody, unconjugated (TMAB-07448) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
IHC-P: 1:200-1000; WB: 1:500-2000; IHC-Fr: 1:200-1000; IF: 1:200-1000
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasmic
ConstructionHybridoma Monoclonal Antibody
AppearanceLiquid
Formulation0.01M PBS (pH7.4).
Research BackgroundAll five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
Antigen Details
Immunogen
Purified native protein
Antigen Species
Human
Gene Name
IGLC1
Gene ID
Protein Name
Immunoglobulin lambda constant 1
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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