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Anti-Human Lambda Light Chain Antibody (8H783) is a Mouse antibody targeting Human Lambda Light Chain. Anti-Human Lambda Light Chain Antibody (8H783) can be used in IHC-P,WB,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
| Description | Anti-Human Lambda Light Chain Antibody (8H783) is a Mouse antibody targeting Human Lambda Light Chain. Anti-Human Lambda Light Chain Antibody (8H783) can be used in IHC-P,WB,IHC-Fr,IF. |
| Ig Type | IgG1 |
| Clone | 8H783 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (TMAB-07448) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (TMAB-07448) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 3. 25 μg total protein per lane of various lysates (see on figure) probed with Human Lambda Light Chain monoclonal antibody, unconjugated (TMAB-07448) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | IHC-P: 1:200-1000; WB: 1:500-2000; IHC-Fr: 1:200-1000; IF: 1:200-1000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasmic |
| Construction | Hybridoma Monoclonal Antibody |
| Appearance | Liquid |
| Formulation | 0.01M PBS (pH7.4). |
| Research Background | All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. |
| Immunogen | Purified native protein |
| Antigen Species | Human |
| Gene Name | IGLC1 |
| Gene ID | |
| Protein Name | Immunoglobulin lambda constant 1 |
| Uniprot ID |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
| Size | Quantity | Unit Price | Amount | Operation |
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