Anti-Human IL-6 Antibody (2G255) is a Mouse antibody targeting Human IL-6. Anti-Human IL-6 Antibody (2G255) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

2G255

Human

1. HUVEC (H) cells were treated with or without LPS (0.5 μg/ml，24h) and Brefeldin A (300 ng/ml，20h), 25 μg total protein per lane of cell lysates (see on figure) probed with human IL-6 monoclonal antibody, unconjugated (TMAB-07446) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human IL-6 Monoclonal Antibody, Unconjugated (TMAB-07446) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

WB: 1:2000-20000; IHC-P: 1:200-800; IHC-Fr: 1:200-800; IF: 1:200-800

Monoclonal

Recombinant Protein: human IL-6 protein

Human

IL6

Interleukin-6

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.