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Anti-Human Ig Kappa light chain Antibody (3X701)

Catalog No. TMAB-07434 Copy Product Info
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Anti-Human Ig Kappa light chain Antibody (3X701) is a Rabbit antibody targeting Human Ig Kappa light chain. Anti-Human Ig Kappa light chain Antibody (3X701) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Human Ig Kappa light chain Antibody (3X701)

Copy Product Info
🥰Excellent
Catalog No. TMAB-07434

Anti-Human Ig Kappa light chain Antibody (3X701) is a Rabbit antibody targeting Human Ig Kappa light chain. Anti-Human Ig Kappa light chain Antibody (3X701) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Human Ig Kappa light chain Antibody
(3X701)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1507-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4727-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Human Ig Kappa light chain Antibody (3X701) is a Rabbit antibody targeting Human Ig Kappa light chain. Anti-Human Ig Kappa light chain Antibody (3X701) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
3X701
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Ig Kappa light chain Monoclonal Antibody, Unconjugated (TMAB-07434) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. 25 μg total protein per lane of various lysates (see on figure) probed with Human Ig kappa light chain monoclonal antibody, unconjugated (TMAB-07434) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasmic and Secreted.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
Antigen Details
Immunogen
A synthesized peptide: human Human Ig kappa light chain
Antigen Species
Human
Gene Name
Igkc
Gene ID
Protein Name
Immunoglobulin kappa constant
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 11 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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