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Anti-human CD90/Thy-1 Antibody (4K978) is a Mouse antibody targeting human CD90/Thy-1. Anti-human CD90/Thy-1 Antibody (4K978) can be used in FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μg | $353 | 7-10 days | 7-10 days |
| Description | Anti-human CD90/Thy-1 Antibody (4K978) is a Mouse antibody targeting human CD90/Thy-1. Anti-human CD90/Thy-1 Antibody (4K978) can be used in FCM. |
| Ig Type | Mouse IgG1 |
| Clone | 4K978 |
| Reactivity | Human |
| Verified Activity | Blank control: HepG2. Primary Antibody (green line): Mouse Anti-CD90 antibody (TMAB-07423) Dilution: 2 μg /10^6 cells; Isotype Control Antibody (orange line): Mouse IgG. Secondary Antibody: Goat anti-Mouse IgG-CY5 Dilution: 1 μg /test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
| Tissue Specificity | Abundant in lymphoid tissues. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M PBS (pH7.4). |
| Concentration | 0.5mg/ml |
| Research Background | Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation. |
| Immunogen | HEL cells |
| Gene Name | THY1 |
| Gene ID | |
| Protein Name | Thy-1 membrane glycoprotein |
| Function | May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. |
| Molecular Weight | Theoretical: 12 kDa. Actual: 25-35 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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