Anti-Human CD63 Antibody (1Y320) is a Mouse antibody targeting Human CD63. Anti-Human CD63 Antibody (1Y320) can be used in FCM,ICC/IF.

Mouse IgG1, k

1Y320

Human

1. 4% Paraformaldehyde-fixed A375 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (human CD63) monoclonal Antibody, unconjugated (TMAB-07416) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The A375 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Mouse Anti-Human CD63 antibody (TMAB-07416): 1 μg/10^6 cells; Isotype Control (orange): Mouse IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM: 1μg/Test; ICC/IF: 1:50-200

Monoclonal

CD63

CD63 antigen

This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.