Anti-human CD4 Antibody (1T334) is a Mouse antibody targeting human CD4. Anti-human CD4 Antibody (1T334) can be used in FCM.

Mouse IgG2b

1T334

Human

Blank control (black line): U937.
Primary Antibody (green line): Mouse Anti-CD4 antibody (TMAB-07407)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Monoclonal

KLH conjugated synthetic peptide: human CD4

Human

CD4

T-cell surface glycoprotein CD4

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.