Anti-human CD30 Antibody (3Z437) is a Mouse antibody targeting human CD30. Anti-human CD30 Antibody (3Z437) can be used in FCM.

Mouse IgG1, k

3Z437

Human

Blank control (black line): sR.
Primary Antibody (green line): Mouse Anti-human CD30 antibody (TMAB-07402)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Monoclonal

TNFRSF8

Tumor necrosis factor receptor superfamily member 8

Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.