Anti-human CD274 (B7-H1, PD-L1) Antibody (9X90) is a Mouse antibody targeting human CD274 (B7-H1, PD-L1). Anti-human CD274 (B7-H1, PD-L1) Antibody (9X90) can be used in FCM.

IgG1

9X90

Human

Blank control (black line): MDA-MB-231.
Primary Antibody (green line): Mouse Anti-Human PD-L1 antibody (TMAB-07398)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Monoclonal

CD274

Programmed cell death 1 ligand 1

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.