Anti-human CD22 Antibody (6Q298) is a Mouse antibody targeting human CD22. Anti-human CD22 Antibody (6Q298) can be used in FCM.

Mouse IgG1

6Q298

Human

1. Blank control: Daudi.
Primary Antibody (green line): Mouse Anti-human CD22 antibody (TMAB-07394)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line) : Goat anti-mouse IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Monoclonal

KLH conjugated synthetic peptide: human CD22

Human

CD22

B-cell receptor CD22

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.