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Anti-human CD11a Antibody (6R990) is a Mouse antibody targeting human CD11a. Anti-human CD11a Antibody (6R990) can be used in ICC/IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μg | $352 | 7-10 days | 7-10 days |
| Description | Anti-human CD11a Antibody (6R990) is a Mouse antibody targeting human CD11a. Anti-human CD11a Antibody (6R990) can be used in ICC/IF,FCM. |
| Ig Type | Mouse IgG2b, k |
| Clone | 6R990 |
| Reactivity | Human |
| Verified Activity | 1. Jurkat cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (human CD11a) monoclonal Antibody, Unconjugated (TMAB-07384) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. 2. scatter diagram showing human peripheral blood leukocytes stained with human CD11a. The cells were incubated with the antibody (TMAB-07384) for 30 min at 22°C. The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed. |
| Application | |
| Recommended Dose | ICC/IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Tissue Specificity | Leukocytes. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4). |
| Research Background | ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Gene Name | ITGAL |
| Gene ID | |
| Protein Name | Integrin alpha-L |
| Function | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. |
| Molecular Weight | Theoretical: 126 kDa. Actual: 180 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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