Anti-Human Bcl-2 Antibody (6Z4) is a Mouse antibody targeting Human Bcl-2. Anti-Human Bcl-2 Antibody (6Z4) can be used in FCM,ICC/IF.

Mouse IgG2b,k

6Z4

Human

1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, unconjugated (TMAB-07382) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Mouse Anti-Bcl-2 antibody (TMAB-07382): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM: 1μg/Test; ICC/IF: 1:100-500

Monoclonal

BCL2

Apoptosis regulator Bcl-2

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).