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Anti-Human Bcl-2 Antibody (6Z4) is a Mouse antibody targeting Human Bcl-2. Anti-Human Bcl-2 Antibody (6Z4) can be used in FCM,ICC/IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μg | $352 | 7-10 days | 7-10 days |
| Description | Anti-Human Bcl-2 Antibody (6Z4) is a Mouse antibody targeting Human Bcl-2. Anti-Human Bcl-2 Antibody (6Z4) can be used in FCM,ICC/IF. |
| Ig Type | Mouse IgG2b,k |
| Clone | 6Z4 |
| Reactivity | Human |
| Application | |
| Recommended Dose | FCM: 1μg/Test; ICC/IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. |
| Tissue Specificity | Expressed in a variety of tissues. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4). |
| Research Background | The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8). |
| Gene Name | BCL2 |
| Gene ID | |
| Protein Name | Apoptosis regulator Bcl-2 |
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). |
| Molecular Weight | Theoretical: 26 kDa. Actual: 26 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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