Anti-HTRA4 Polyclonal Antibody is a Rabbit antibody targeting HTRA4. Anti-HTRA4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Cow)

1. Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-HTRA4 Polyclonal Antibody, Unconjugated (TMAB-07381) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
2. Protein: DU145 (human) lyates at 30 μg;
Primary: Rabbit Anti-HTRA4 at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit Igg (H+L) at 1: 10000;
Predicted band size:49 kD
Observed band size:50 kD
3. Sample: PC-3 Cell (Human) Lysate at 30 μg
Primary: Anti-HTRA4 (TMAB-07381) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
4. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-HTRA4 (TMAB-07381) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 50 kDa
5. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (HTRA4) Polyclonal Antibody, Unconjugated (TMAB-07381) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HTRA4

Human

HTRA4

Serine protease HTRA4

Serine protease.