Anti-HTRA2 Polyclonal Antibody is a Rabbit antibody targeting HTRA2. Anti-HTRA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Cow,Rabbit)

1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-HTRA2 Polyclonal Antibody, Unconjugated (TMAB-07379) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
LOVO Cell Lysate at 40 μg
TT Cell Lysate at 40 μg
Primary: Anti-HTRA2 (TMAB-07379) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
3. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Rat) Lysate at 40 μg
Lane 3: Hela (Human) Cell Lysate at 30 μg
Lane 4: U2os (Human) Cell Lysate at 30 μg
Lane 5: HT1080 (Human) Cell Lysate at 30 μg
Lane 6: Jurkat (Human) Cell Lysate at 30 μg
Primary: Anti-HTRA2 (TMAB-07379) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 32 kD
4. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HTRA2) polyclonal Antibody, Unconjugated (TMAB-07379) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HtrA2

Human

HTRA2

Serine protease HTRA2, mitochondrial

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.