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Anti-HSPB6 Antibody (5V2)
Catalog No. TMAB-07362 Copy Product Info
🥰Excellent
Anti-HSPB6 Antibody (5V2) is a Mouse antibody targeting HSPB6. Anti-HSPB6 Antibody (5V2) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HSPB6 Antibody (5V2)
Copy Product Info🥰Excellent
Catalog No. TMAB-07362
Anti-HSPB6 Antibody (5V2) is a Mouse antibody targeting HSPB6. Anti-HSPB6 Antibody (5V2) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HSPB6 Antibody
(5V2)
(5V2)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-HSPB6 Antibody (5V2) is a Mouse antibody targeting HSPB6. Anti-HSPB6 Antibody (5V2) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG1, Kappa |
| Clone | 5V2 |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Tissue: Human skeletal muscle Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for TMAB-07362 2. Tissue: Mouse colon Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for TMAB-07362 3. Tissue: Rat heart Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for TMAB-07362 4. Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition:4°C overnight Lysate:1: Mouse muscle, 2: Rat heart, 3: C2C12 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 17 kDa Observed MW: 17 kDa |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Translocates to nuclear foci during heat shock. |
| Construction | Recombinant Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and may form different hetercomplexes with these proteins. The specific physiological function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions, Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions, it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. The Hsp20 was demonstrated to constitute up to 1.3% of the total cellular protein in vertebrate tissues, especially in muscle, and its expression is related to muscle contraction, specifically in slow-twitch muscle. Hsp20 may form different heterocomplexes with other Hsp's, such as alpha-crystalline and Hsp25. Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments. In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction. |
| Gene Name | HSPB6 |
| Gene ID | |
| Protein Name | Heat shock protein beta-6 |
| Molecular Weight | Theoretical: 17 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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