Anti-HSPA5 Polyclonal Antibody is a Rabbit antibody targeting HSPA5. Anti-HSPA5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Pig)

1. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GRP78 Polyclonal Antibody, Unconjugated (TMAB-00898) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Spleen (Rat) Lysate at 40 μg
Lane 2: Cerebrum (Rat) Lysate at 40 μg
Lane 3: U-87MG (Human) Cell Lysate at 30 μg
Lane 4: Hela (Human) Cell Lysate at 30 μg
Lane 5: HepG2 (Human) Cell Lysate at 30 μg
Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 μg
Lane 7: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-GRP78 (TMAB-00898) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 74 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GRP78) Polyclonal Antibody, Unconjugated (TMAB-00898) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human GRP78

Human

ER